CAS No. 148-82-3

Batch: Purity:99.47
Melphalan Chemical Structure
Chemical Structure
Check the Melphalan product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-2-amino-3-[4-[bis(2-chloroethyl)amino]phenyl]propanoic acid
CAS Number 148-82-3
Molecular Formula

C13H18Cl2N2O2

Molecular Weight (MW) 305.20
SMILES C1=CC(=CC=C1CC(C(=O)O)N)N(CCCl)CCCl
InChIKey SGDBTWWWUNNDEQ-LBPRGKRZSA-N

Ordering Information

Biological Activity

Melphalan is a phenylalanine derivative of nitrogen mustard that acts as an antineoplastic DNA-alkylating agent. It covalently binds to genomic DNA to form intra- and interstrand cross-links, effectively impairing DNA replication and RNA transcription. This severe DNA damage triggers p53 signaling pathway activation, leading to cell cycle arrest and inducing apoptotic cell death across multiple tumor cell lines.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 3.5 mg/mL (11.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.