CAS No. 2549174-42-5

Batch: Purity:99.01
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Lirafugratinib (RLY-4008) Chemical Structure
Chemical Structure
Check the Lirafugratinib (RLY-4008) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[4-[4-amino-5-[3-fluoro-4-(4-methylpyrimidin-2-yl)oxyphenyl]-7-methylpyrrolo[2,3-d]pyrimidin-6-yl]phenyl]-2-methylprop-2-enamide
CAS Number 2549174-42-5
Molecular Formula

C28H24FN7O2
Molecular Weight (MW) 509.54
InChIKey XOQVZSSDIQQUGO-UHFFFAOYSA-N

Biological Activity

Lirafugratinib (RLY-4008) is an orally active, irreversible, and selective inhibitor of fibroblast growth factor receptor 2 (FGFR2) that exhibits >250-fold selectivity over FGFR1 and >5,000-fold selectivity over FGFR4. By selectively targeting wild-type and mutated FGFR2, it impairs downstream receptor tyrosine kinase signaling pathways. Consequently, lirafugratinib inhibits cellular proliferation and drives tumor regression in preclinical cancer models driven by primary FGFR2 alterations or resistance mutations. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 20 mg/mL (39.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 2 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Subbiah V, et al. Cancer Discov. 2023;13(9):2012-2031.

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Note: Technical data last updated: Sep 1, 2026.