CAS No. 2110428-64-1

Batch: Purity:99.39
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JNJ-63576253 (TRC-253) Chemical Structure
Chemical Structure
Check the JNJ-63576253 (TRC-253) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 2110428-64-1
Molecular Formula

C23H22ClF3N6O2S
Molecular Weight (MW) 538.97
SMILES Cl.FC(F)(F)C1=CC(=CN=C1C#N)N2C(=O)C3(CCC3)N(C2=S)C4=CN=C(OC5CCNCC5)C=C4

Ordering Information

Biological Activity

JNJ-63576253 (TRC-253) is a potent and selective androgen receptor (AR) antagonist designed to overcome antiandrogen resistance. It inhibits both wild-type AR and ligand-binding domain-mutated AR variants associated with enzalutamide resistance. By blocking AR nuclear translocation and transcriptional activity, JNJ-63576253 suppresses androgen-responsive gene expression, disrupting downstream AR pathway signaling and impairing cell proliferation in prostate cancer models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (185.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.