CAS No. 144-48-9
Batch:
Purity:97
Chemical Structure
Check the
Iodoacetamide
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 2-iodoacetamide | |
|---|---|---|
| CAS Number | 144-48-9 | |
| Molecular Formula |
C2H4INO |
|
| Molecular Weight (MW) | 184.96 | |
| InChIKey | PGLTVOMIXTUURA-UHFFFAOYSA-N |
Iodoacetamide is an irreversible alkylating agent that covalently modifies nucleophilic cysteine thiol groups in proteins. By carbamidomethylating free sulfhydryl groups, it prevents the reformation of disulfide bonds and stabilizes reduced protein conformations. This alkylation mechanism disrupts cysteine-dependent enzymatic activities and is widely utilized in proteomic workflows to facilitate protein denaturation and enzymatic digestion prior to mass spectrometry analysis. [1]
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
40 mg/mL
(216.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 40 mg/mL |
| Ethanol | 40 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.