CAS No. 16009-13-5

Batch: Purity:98
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Hemin Chemical Structure
Chemical Structure
Check the Hemin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 16009-13-5
Molecular Formula

C34H32ClFeN4O4
Molecular Weight (MW) 651.94
SMILES CC1=C(C2=CC3=C(C(=C([N-]3)C=C4C(=C(C(=N4)C=C5C(=C(C(=N5)C=C1[N-]2)C)C=C)C)C=C)C)CCC(=O)O)CCC(=O)O.[Cl-].[Fe+3]

Ordering Information

Biological Activity

Hemin is an iron-containing porphyrin that acts as a potent inducer of heme oxygenase-1 (HO-1). Up-regulation of HO-1 by hemin enhances the catabolism of free heme into carbon monoxide, free iron, and biliverdin, thereby attenuating oxidative stress and exerting cytoprotective as well as anti-inflammatory activities across various cellular and tissue models.

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 20 mg/mL (30.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.