CAS No. 2799-07-7

Batch: Purity:98
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H-Cys(Trt)-OH Chemical Structure
Chemical Structure
Check the H-Cys(Trt)-OH product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2R)-2-amino-3-tritylsulfanylpropanoic acid
CAS Number 2799-07-7
Molecular Formula

C22H21NO2S
Molecular Weight (MW) 363.47
SMILES C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SCC(C(=O)O)N
InChIKey DLMYFMLKORXJPO-FQEVSTJZSA-N

Biological Activity

H-Cys(Trt)-OH (S-Trityl-L-cysteine) is a specific inhibitor of the kinesin spindle protein Eg5 that reversibly inhibits Eg5-driven microtubule sliding velocity with an IC50 of 500 nM. By targeting Eg5, it disrupts mitotic spindle bipolarity and blocks chromosome segregation during mitosis. This inhibition leads to cell cycle arrest at the G2/M phase and induces apoptotic cell death in cancer cells.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 5 mg/mL (13.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.