CAS No. 58-05-9
Batch:
Purity:99.70
Chemical Structure
Check the
Folinic acid (Leucovorin)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 58-05-9 | |
|---|---|---|
| Molecular Formula |
C20H23N7O7 |
|
| Molecular Weight (MW) | 473.44 |
Folinic acid (Leucovorin) is a reduced folate metabolite that expands intracellular pools of reduced folates. It serves as a precursor to 5,10-methylenetetrahydrofolate, which stabilizes the ternary complex formed with thymidylate synthase (TS) and fluorodeoxyuridylate (FdUMP). This enhances the downstream inhibition of thymidylate synthase, leading to suppressed pyrimidine biosynthesis, impaired DNA synthesis, and inhibition of cellular proliferation.
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
180 mg/mL
(380.19 mM)
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
95 mg/mL
(200.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.