CAS No. 73231-34-2

Batch: Purity:99.04
Florfenicol Chemical Structure
Chemical Structure
Check the Florfenicol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 73231-34-2
Molecular Formula

C12H14Cl2FNO4S

Molecular Weight (MW) 358.21
SMILES CS(=O)(=O)C1=CC=C(C=C1)C(C(CF)NC(=O)C(Cl)Cl)O

Ordering Information

Biological Activity

Florfenicol is a fluorinated synthetic analog of thiamphenicol that acts as a broad-spectrum antibacterial agent. It selectively binds to the 50S ribosomal subunit in susceptible bacteria, inhibiting peptidyl transferase activity and blocking peptide bond formation. This inhibition impairs bacterial protein translation, resulting in bacteriostatic growth suppression across a wide variety of Gram-positive and Gram-negative pathogens. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 72 mg/mL (200.99 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 8 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.