CAS No. 159351-69-6

Batch: Purity:99.78
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RAD001 (Everolimus) Chemical Structure
Chemical Structure
Check the RAD001 (Everolimus) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1S,3R,4R)-4-(2-hydroxyethoxy)-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone
CAS Number 159351-69-6
Molecular Formula

C53H83NO14
Molecular Weight (MW) 958.22
SMILES CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)OCCO)C)C)O)OC)C)C)C)OC
InChIKey HKVAMNSJSFKALM-GKUWKFKPSA-N

Ordering Information

Biological Activity

Everolimus (RAD001) is a potent mTOR inhibitor that binds FKBP-12 to form an inhibitory complex, demonstrating an IC50 ofin cell-free assays. This inhibition suppresses downstream AKT/mTOR signaling pathways, leading to blockades in T-cell activation and cytokine-stimulated cell proliferation. Furthermore, Everolimus induces apoptosis and autophagy while effectively inhibiting tumor cell growth in various human cancer models. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (104.36 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.