CAS No. 60-00-4

Batch: Purity:99
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Ethylenediaminetetraacetic acid Chemical Structure
Chemical Structure
Check the Ethylenediaminetetraacetic acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid
CAS Number 60-00-4
Molecular Formula
C10H16N2O8
Molecular Weight (MW) 292.24
InChIKey KCXVZYZYPLLWCC-UHFFFAOYSA-N

Biological Activity

Ethylenediaminetetraacetic acid (EDTA) is a polyamino carboxylic acid and chelating agent that sequesters divalent metal ions, including calcium, magnesium, zinc, and iron. By binding essential metal cofactors, EDTA suppresses metalloprotease activity and inhibits metal ion-catalyzed oxidative damage to proteins. Furthermore, chelation of calcium and magnesium disrupts complement activation cascades and prevents unwanted disulfide bond formation, thereby preserving protein structural integrity and reducing background signal in immunoassays. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
4-Methylpyridine 5 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Auld DS. Biometals. 2001;14(3-4):271-313.

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Note: Technical data last updated: Sep 1, 2026.