CAS No. 56296-18-5

Batch: Purity:99.80
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DREADD agonist 21 Chemical Structure
Chemical Structure
Check the DREADD agonist 21 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 6-piperazin-1-yl-11H-benzo[b][1,4]benzodiazepine
CAS Number 56296-18-5
Molecular Formula

C17H18N4
Molecular Weight (MW) 278.35
SMILES C1CN(CCN1)C2=NC3=CC=CC=C3NC4=CC=CC=C42
InChIKey JCBYXNSOLUVGTF-UHFFFAOYSA-N

Biological Activity

DREADD agonist 21 is a potent synthetic agonist of human muscarinic M3 DREADD receptors (hM3Dq, EC50 =. Activation of hM3Dq engages Gq-protein coupled signaling pathways, triggering intracellular calcium mobilization and neuronal excitation. This compound enables precise, temporally controlled chemogenetic modulation of specific neuronal populations to elucidate neural circuit dynamics and behavioral functions.

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 56 mg/mL (201.18 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 56 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.