CAS No. 58537-94-3

Batch: Purity:99.00
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Dimethyl pimelimidate dihydrochloride Chemical Structure
Chemical Structure
Check the Dimethyl pimelimidate dihydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 58537-94-3
Molecular Formula

C9H18N2O2.2ClH
Molecular Weight (MW) 259.17

Ordering Information

Biological Activity

Dimethyl pimelimidate dihydrochloride is a homobifunctional amine-reactive crosslinker that targets primary amine groups on lysine residues within proteins. The compound contains imidoester functional groups at both ends of a 7-carbon spacer arm, reacting at alkaline pH to form stable amidine bonds. Covalent crosslinking of proteins stabilizes protein complexes and covalently immobilizes antibodies onto solid supports, facilitating efficient immunoprecipitation and affinity purification assays. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 45 mg/mL (173.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 45 mg/mL
Ethanol 45 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.