Cell-free Assays (IC50/Ki) for Cyclosporin A
| Key Target | IC50 (Cell-free) | Description | Reference |
|---|---|---|---|
| rat liver mitochondria | 34 nM | Ca2+ retention capacity (CRC) assay (using Fluo-4FF fluorescence) | Download PDF |
| HeLa S3 mitochondria | 120 nM | Ca2+ retention capacity (CRC) assay (using Fluo-4FF fluorescence) | Download PDF |
| recombinant human CypD protein | 23 nM | CsA/CypD homogenous time-resolved fluorescence (HTRF) assay | Download PDF |
| CypA | 8.6 ± 1.2 nM | Liquid chromatography-based high-throughput PPIase activity assay | Download PDF |
Cell-based Assay (IC50) for Cyclosporin A
| Cell Type | IC50 (Cell-based) | Description | Reference |
|---|---|---|---|
| Jurkat T cells | 120.5 nM | IL-2 release measurement | Download PDF |
| Primary human hepatocytes | 0.91 ± 0.19 µM | HBe antigen quantification | PMC7172969 |
| HepG2-hNTCP-C4 cells | 1.49 ± 0.10 µM | Taurocholic acid (TCA) uptake activity measurement | PMC7172969 |
| MPTP | 5 nM | MPTP inhibition | PMC7379599 |
| RAW 264.7 cells | 123.34 ± 17.45 µg/ml | MTT assay | PMC12135089 |
| mouse precision-cut liver slices | 9.5 µM | ATP-luminescence assay, nonlinear regression analysis of the best-curve fit | Download PDF |
| OATP1B1 | 2.25 μM | OATP1B1-mediated MPAG uptake inhibition assay, quantified by LC-MS, calculated by nonlinear regression based on a three-parameter logistic function. | Download PDF |
| OATP1B3 | 0.906 μM | OATP1B3-mediated MPAG uptake inhibition assay, quantified by LC-MS, calculated by nonlinear regression based on a three-parameter logistic function. | Download PDF |
| HEK293-OATP1B1 cells | 1.88 μM | UPLC-MS/MS analysis of intracellular atorvastatin content | PMC3264873 |
| HMEC-1 | 3.286 µM | resazurin reduction assay | Download PDF |
| MCF-7/ADR | 0.49 μM | P-glycoprotein inhibition assay (ADM efflux) | Download PDF |
Check the
Cyclosporin A
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
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