CAS No. 6055-19-2

Batch: Purity:98.29
Cyclophosphamide Monohydrate Chemical Structure
Chemical Structure
Check the Cyclophosphamide Monohydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N,N-bis(2-chloroethyl)-2-oxo-1,3,2λ5-oxazaphosphinan-2-amine;hydrate
CAS Number 6055-19-2
Molecular Formula

C7H15Cl2N2O2P.H2O

Molecular Weight (MW) 279.1
SMILES C1CNP(=O)(OC1)N(CCCl)CCCl.O
InChIKey PWOQRKCAHTVFLB-UHFFFAOYSA-N

Ordering Information

Biological Activity

Cyclophosphamide Monohydrate is a nitrogen mustard alkylating agent that targets genomic DNA by attaching alkyl groups to guanine bases. This alkylation leads to DNA crosslinking, strand breakage, and induction of mutations, ultimately inhibiting DNA replication and triggering cell death. Additionally, Cyclophosphamide Monohydrate is widely utilized to induce animal models of anemia and bone marrow suppression. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 55 mg/mL (197.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 55 mg/mL
Ethanol 55 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.

Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.

Note: Technical data last updated: Sep 1, 2026.