CAS No. 55-56-1

Batch: Purity:99.8
Chlorhexidine Chemical Structure
Chemical Structure
Check the Chlorhexidine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 55-56-1
Molecular Formula

C22H30Cl2N10

Molecular Weight (MW) 505.45
SMILES C1=CC(=CC=C1NC(=NC(=NCCCCCCN=C(N)N=C(N)NC2=CC=C(C=C2)Cl)N)N)Cl

Ordering Information

Biological Activity

Chlorhexidine is a cationic bisbiguanide disinfectant and topical anti-infective agent. It exhibits broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, facultative anaerobes, aerobes, and yeasts. Chlorhexidine binds to negatively charged bacterial cell wall components, causing disruption of the cytoplasmic membrane. This disruption leads to the leakage of intracellular constituents, inhibition of membrane-bound enzymes, and ultimate cell lysis, thereby exerting bactericidal and fungicidal effects. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (197.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.