CAS No. 150347-59-4

Batch: Purity:99.85
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CFSE (CFDA-SE) Chemical Structure
Chemical Structure
Check the CFSE (CFDA-SE) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate;(2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
CAS Number 150347-59-4
Molecular Formula

C29H19NO11
Molecular Weight (MW) 557.46
SMILES CC(=O)OC1=CC=C2C(=C1)OC3=C(C=CC(=C3)OC(C)=O)C24OC(=O)C5=CC(=CC=C45)C(=O)ON6C(=O)CCC6=O.CC(=O)OC7=CC=C8C(=C7)OC9=C(C=CC(=C9)OC(C)=O)C8%10OC(=O)C%11=CC=C(C=C%10%11)C(=O)ON%12C(=O)CCC%12=O
InChIKey LZFDYQHOQDFMOA-UHFFFAOYSA-N

Ordering Information

Biological Activity

CFSE (CFDA-SE) is a cell-permeable fluorescent staining dye widely used to track cell proliferation and motility. Upon entering cells, cleavage by intracellular esterases yields amine-reactive fluorescein derivatives that covalently couple via succinimidyl groups to intracellular lysine residues and proteins. The resulting fluorescent conjugates are stably retained within living cells and partitioned equally between daughter cells during division, enabling tracking of cell division kinetics by flow cytometry. [1][2]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (179.38 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.