CAS No. 58-71-9

Batch: Purity:99.88
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Cephalothin sodium Chemical Structure
Chemical Structure
Check the Cephalothin sodium product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name sodium (6R,7R)-3-(acetyloxymethyl)-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate
CAS Number 58-71-9
Molecular Formula

C16H15N2NaO6S2
Molecular Weight (MW) 418.42
SMILES CC(=O)OCC1=C(N2C(C(C2=O)NC(=O)CC3=CC=CS3)SC1)C(=O)[O-].[Na+]
InChIKey VUFGUVLLDPOSBC-XRZFDKQNSA-M

Biological Activity

Cephalothin sodium is a first-generation, beta-lactam cephalosporin antibiotic that targets penicillin-binding proteins (PBPs) located on the inner membrane of the bacterial cell wall. Inactivation of PBPs disrupts peptidoglycan chain cross-linking during cell wall synthesis, compromising structural integrity. This inhibition leads to osmotic instability, cell lysis, and bactericidal activity against susceptible bacterial strains.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 83 mg/mL
DMSO 10 mg/mL (23.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.