Molecular Weight: 622.53 g/mol

Calcein Chemical Structure
Chemical Structure
Check the Calcein product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 50 mg/mL (80.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Chemical Information

CAS Number 1461-15-0
Molecular Formula

C30H26N2O13
Molecular Weight (MW) 622.53
SMILES OC(=O)CN(CC(O)=O)CC1=CC2=C(OC3=C(C=C(CN(CC(O)=O)CC(O)=O)C(=C3)O)C24OC(=O)C5=CC=CC=C45)C=C1O

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Note: Technical data last updated: Sep 1, 2026.