CAS No. 1461-15-0
Batch:
Purity:99.58
Chemical Structure
Check the
Calcein
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 1461-15-0 | |
|---|---|---|
| Molecular Formula |
C30H26N2O13 |
|
| Molecular Weight (MW) | 622.53 | |
| SMILES | OC(=O)CN(CC(O)=O)CC1=CC2=C(OC3=C(C=C(CN(CC(O)=O)CC(O)=O)C(=C3)O)C24OC(=O)C5=CC=CC=C45)C=C1O |
Calcein (Fluorexon) is a cell-impermeable fluorescent fluorophore and fluid-phase marker used to track and visualize cellular processes such as synaptic vesicle fusion and membrane dynamics. As a hydrophilic fluorescent dye, it serves as a reporter for membrane integrity, cell viability, and liposomal fusion or leakage. When introduced via esterase-cleavable precursors or microinjection, trapped intracellular Calcein emits strong green fluorescence, enabling live-cell labeling and tracking of cytosolic events. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
50 mg/mL
(80.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.