CAS No. 20350-15-6
| IUPAC Name | (1R,2R,3E,7S,11E,13S,15S)-2,15-dihydroxy-7-methyl-6-oxabicyclo[11.3.0]hexadeca-3,11-dien-5-one | |
|---|---|---|
| CAS Number | 20350-15-6 | |
| Molecular Formula |
C16H24O4 |
|
| Molecular Weight (MW) | 280.36 | |
| SMILES | CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O | |
| InChIKey | KQNZDYYTLMIZCT-KQPMLPITSA-N |
Brefeldin A is a lactone antibiotic and inhibitor of protein transport that targets intracellular vesicular trafficking. It disrupts Golgi complex structure, blocks endoplasmic reticulum-to-Golgi transport, and triggers endoplasmic reticulum stress, ultimately inducing cancer cell differentiation and apoptosis. Furthermore, Brefeldin A inhibits autophagy and mitophagy, and serves as a small-molecule enhancer to improve CRISPR-mediated homology-directed repair efficiency in gene editing applications. [1]
|
Concentration
Volume
Mass
|
|---|
| Solvent | Solubility & Note |
|---|---|
| DMSO |
125 mg/mL
(445.85 mM)
; Warmed with 60°C water bath
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
56 mg/mL
(199.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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