Cell-free Assays (IC50/Ki) for (-)-Blebbistatin
| Key Target | IC50 (Cell-free) | Description | Reference |
|---|---|---|---|
| Human myosin IIA ATPase | 5.1 μM | Inhibition of ATPase activity | Download PDF |
| rabbit skeletal muscle myosin II | 2.16 µM | in-house developed ATPase inhibitory activity assay | Download PDF |
| Myh7b | 0.36 μM | NADH-coupled assay, Hill equation fit | Download PDF |
| β-cardiac myosin S1 | 1.12 μM | NADH-coupled assay, Hill equation fit | Download PDF |
| rabbit skeletal muscle myosin S1 | 0.3 ± 0.03 μM (basal ATPase), 0.11 ± 0.009 μM (actin activat | Pyruvate kinase/lactate dehydrogenase coupled assay (NADH-coupled assay) | Download PDF |
| Dictyostelium discoideum myosin II motor domain | 4.4 ± 0.3 μM (basal ATPase), 3.9 ± 0.3 μM (actin activated A | Pyruvate kinase/lactate dehydrogenase coupled assay (NADH-coupled assay) | Download PDF |
| Rabbit fast muscle actin and HMM | ~1–5 mM | in vitro motility assay (sliding velocity) | Download PDF |
Cell-based Assay (IC50) for (-)-Blebbistatin
| Cell Type | IC50 (Cell-based) | Description | Reference |
|---|---|---|---|
| HeLa cells | 6.4 ± 4.8 μM | Cell number quantification | Download PDF |
Check the
(-)-Blebbistatin
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
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