CAS No. 2241337-84-6

Batch: Purity:99.37
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Bemnifosbuvir Hemisulfate (AT-527) Chemical Structure
Chemical Structure
Check the Bemnifosbuvir Hemisulfate (AT-527) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name bis(propan-2-yl (2S)-2-[[[(2R,3R,4R,5R)-5-[2-amino-6-(methylamino)purin-9-yl]-4-fluoro-3-hydroxy-4-methyloxolan-2-yl]methoxy-phenoxyphosphoryl]amino]propanoate);sulfuric acid
CAS Number 2241337-84-6
Molecular Formula

C24H33FN7O7P.1/2H2O4S
Molecular Weight (MW) 630.55
SMILES CNC1=C2N=C[N](C3OC(CO[P](=O)(NC(C)C(=O)OC(C)C)OC4=CC=CC=C4)C(O)C3(C)F)C2=NC(=N1)N.O[S](O)(=O)=O
InChIKey QIGYBLSWYRTXCA-NVSCJZCKSA-N

Biological Activity

Bemnifosbuvir hemisulfate is a guanosine nucleotide prodrug targeting viral RNA-dependent RNA polymerase, whose free base form AT-511 inhibits SARS-CoV-2 replication with an EC90 of 0.47 μM in human airway epithelial cells. Upon intracellular conversion to its active triphosphate metabolite, it incorporates into viral RNA, leading to premature termination of transcription and blockade of viral replication pathways. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (158.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 100 mg/mL
Ethanol 100 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Good SS, et al. Antimicrob Agents Chemother. 2021;65(4).

[2] Shannon A, et al. Nat Commun. 2022;13(1):621.

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Note: Technical data last updated: Sep 1, 2026.