SMILES:

N[S](=O)(=O)C1=CC(=C(NCC2=CC=CS2)C=C1Cl)C3=N[NH]N=N3
Azosemide Chemical Structure
Chemical Structure
Check the Azosemide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-chloro-5-(2H-tetrazol-5-yl)-4-(thiophen-2-ylmethylamino)benzenesulfonamide
CAS Number 27589-33-9
Molecular Formula

C12H11ClN6O2S2
Molecular Weight (MW) 370.84
SMILES N[S](=O)(=O)C1=CC(=C(NCC2=CC=CS2)C=C1Cl)C3=N[NH]N=N3
InChIKey HMEDEBAJARCKCT-UHFFFAOYSA-N

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 74 mg/mL (199.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.