CAS No. 54301-15-4

Batch: Purity:99.99
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Amsacrine hydrochloride Chemical Structure
Chemical Structure
Check the Amsacrine hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[4-(acridin-9-ylamino)-3-methoxyphenyl]methanesulfonamide;hydrochloride
CAS Number 54301-15-4
Molecular Formula

C21H20ClN3O3S
Molecular Weight (MW) 429.92
SMILES COC1=C(C=CC(=C1)NS(=O)(=O)C)NC2=C3C=CC=CC3=NC4=CC=CC=C42.Cl
InChIKey WDISRLXRMMTXEV-UHFFFAOYSA-N

Biological Activity

Amsacrine hydrochloride is an acridine derivative and potent inhibitor of DNA topoisomerase II with antineoplastic activity. It intercalates into DNA and stabilizes topoisomerase II-DNA cleavable complexes, inducing double-strand DNA breaks and inhibiting DNA replication and transcription. This accumulation of DNA damage triggers apoptotic pathways and arrests cell cycle progression, suppressing tumor cell proliferation.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 85 mg/mL (197.71 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.