Afatinib is an irreversible ErbB inhibitor. It affects AKT/ERK signaling pathways, by covalent binding, inhibiting cell growth.
Afatinib (BIBW2992) IC50 for Cell-based and Cell-free Assays
Comprehensive IC50 Values for Afatinib (BIBW2992) Across Cell-free Enzyme Inhibitory Profiles and Cell-based Functional Assays.