CAS No. 850140-72-6
Batch:
Purity:99.95
Chemical Structure
Check the
Afatinib (BIBW2992)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (E)-N-[4-(3-chloro-4-fluoroanilino)-7-[(3S)-oxolan-3-yl]oxyquinazolin-6-yl]-4-(dimethylamino)but-2-enamide | |
|---|---|---|
| CAS Number | 850140-72-6 | |
| Molecular Formula |
C24H25ClFN5O3 |
|
| Molecular Weight (MW) | 485.94 | |
| SMILES | CN(C)CC=CC(=O)NC1=C(C=C2C(=C1)C(=NC=N2)NC3=CC(=C(C=C3)F)Cl)OC4CCOC4 | |
| InChIKey | ULXXDDBFHOBEHA-CWDCEQMOSA-N |
Biological Activity
Afatinib (BIBW2992) is an irreversible tyrosine kinase inhibitor targeting EGFR wt, EGFR L858R, EGFR L858R/T790M, ErbB2, and ErbB4 (IC50 1 nM). It suppresses receptor autophosphorylation and downstream AKT and ERK signaling pathways, leading to inhibition of cellular proliferation and induction of autophagy and apoptosis in carcinoma cells.
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
97 mg/mL
(199.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 97 mg/mL |
| Water | Insoluble |
| DMSO |
97 mg/mL
(199.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 90 mg/mL |
| Water | Insoluble |
| DMSO |
250 mg/mL
(514.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 97 mg/mL |
| Water | Insoluble |
| DMSO |
97 mg/mL
(199.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 97 mg/mL |
| Water | Insoluble |
| DMSO |
97 mg/mL
(199.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 97 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Jump to
- Afatinib (BIBW2992) Mechanism of Action: EGFR Inhibition in Lung Adenocarcinoma Cell Research
- Afatinib (BIBW2992) Clinical Trials
- Afatinib (BIBW2992) Working Concentration | Cell Culture | Treatment
- Afatinib (BIBW2992) Solubility in DMSO
- Afatinib (BIBW2992) Stock Solution
- Afatinib (BIBW2992) Storage
- Afatinib (BIBW2992) Stability
- Afatinib (BIBW2992) Combination Database
- Afatinib (BIBW2992) Molecular Weight
- Afatinib (BIBW2992) SMILES
- Afatinib (BIBW2992) Chemical Structure (2D and 3D)
- Afatinib (BIBW2992) IC50 for Cell-based and Cell-free Assays
- Afatinib (BIBW2992) MedChemExpress (HY-10261)? Try a better option.
- Afatinib (BIBW2992) Sigma (SML3109)? Try a better option.
- Afatinib (BIBW2992) Cayman (11492)? Try a better option.
- Afatinib (BIBW2992) APExBIO (A4746)? Try a better option.
- Afatinib (BIBW2992) SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.