CAS No. 844499-71-4
Batch:
Purity:99.67
Chemical Structure
Check the
A-769662
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 4-hydroxy-3-[4-(2-hydroxyphenyl)phenyl]-6-oxo-7H-thieno[2,3-b]pyridine-5-carbonitrile | |
|---|---|---|
| CAS Number | 844499-71-4 | |
| Molecular Formula |
C20H12N2O3S |
|
| Molecular Weight (MW) | 360.39 | |
| SMILES | C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O | |
| InChIKey | CTESJDQKVOEUOY-UHFFFAOYSA-N |
Biological Activity
A-769662 is a potent, reversible AMP-activated protein kinase (AMPK) activator with an EC50 of 0.8 μM. It selectively activates β1 subunit-containing AMPK heterotrimers, promoting downstream phosphorylation of acetyl-CoA carboxylase (ACC) and inhibiting fatty acid synthesis with an IC50 of 3.2 μM. Activation of this pathway modulates cellular lipid metabolism, leading to reduced plasma glucose and triglyceride levels in vivo. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(277.47 mM)
; Warmed with 60°C water bath
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
72 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
72 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
72 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
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Note: Technical data last updated: Sep 1, 2026.