CAS No. 32986-56-4

Batch: Purity:99.87
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Tobramycin Chemical Structure
Chemical Structure
Check the Tobramycin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S,3R,4S,5S,6R)-4-amino-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2R,3R,5S,6R)-3-amino-6-(aminomethyl)-5-hydroxyoxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-6-(hydroxymethyl)oxane-3,5-diol
CAS Number 32986-56-4
Molecular Formula

C18H37N5O9
Molecular Weight (MW) 467.52369
SMILES C1C(C(C(C(C1N)OC2C(C(C(C(O2)CO)O)N)O)O)OC3C(CC(C(O3)CN)O)N)N
InChIKey NLVFBUXFDBBNBW-PBSUHMDJSA-N

Ordering Information

Biological Activity

Tobramycin is an aminoglycoside antibiotic that targets the bacterial 30S ribosomal subunit. By specifically binding to the A-site of 16S ribosomal RNA, it causes mRNA mistranslation and inhibits protein synthesis in bacterial cells. This interference disrupts essential cellular pathways, leading to bactericidal cell death, and exhibits potent antibacterial activity against Gram-negative pathogens, including Pseudomonas aeruginosa. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 94 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.