CAS No. 154-42-7

Batch: Purity:99.93
Check CoA
Thioguanine Chemical Structure
Chemical Structure
Check the Thioguanine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-amino-3,7-dihydropurine-6-thione
CAS Number 154-42-7
Molecular Formula

C5H5N5S
Molecular Weight (MW) 167.19
SMILES C1=NC2=C(N1)C(=S)N=C(N2)N
InChIKey WYWHKKSPHMUBEB-UHFFFAOYSA-N

Ordering Information

Biological Activity

Thioguanine is a purine antimetabolite that functions as an inhibitor of DNA methyltransferase 1 (DNMT1). It promotes ubiquitin-targeted degradation of DNMT1, leading to reduced DNA methylation and alterations in epigenomic regulation. Incorporating into cellular DNA and RNA, thioguanine disrupts nucleic acid synthesis, triggers DNA mismatch repair responses, and inhibits cell proliferation in mammalian cell lines.

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 17 mg/mL (101.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.