CAS No. 3056-17-5

Batch: Purity:99.74
d4T (Stavudine) Chemical Structure
Chemical Structure
Check the d4T (Stavudine) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2R,5S)-5-(hydroxymethyl)-2,5-dihydrofuran-2-yl]-5-methylpyrimidine-2,4-dione
CAS Number 3056-17-5
Molecular Formula

C10H12N2O4

Molecular Weight (MW) 224.21
SMILES CC1=CN(C(=O)NC1=O)C2C=CC(O2)CO
InChIKey XNKLLVCARDGLGL-JGVFFNPUSA-N

Ordering Information

Biological Activity

Stavudine (d4T) is a thymidine nucleoside analog reverse transcriptase inhibitor (NARTI) that targets viral reverse transcriptase. Intracellular kinases phosphorylate stavudine to its active form, stavudine triphosphate, which competes with natural thymidine triphosphate for binding to HIV reverse transcriptase. Incorporation of stavudine triphosphate into nascent viral cDNA causes DNA chain termination, thereby effectively disrupting viral genome synthesis and blocking HIV replication. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 49 mg/mL (218.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 24 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.