CAS No. 280744-09-4
Batch:
Purity:99.98
Chemical Structure
Check the
SB216763
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 3-(2,4-dichlorophenyl)-4-(1-methylindol-3-yl)pyrrole-2,5-dione | |
|---|---|---|
| CAS Number | 280744-09-4 | |
| Molecular Formula |
C19H12N2O2Cl2 |
|
| Molecular Weight (MW) | 371.22 | |
| SMILES | CN1C=C(C2=CC=CC=C21)C3=C(C(=O)NC3=O)C4=C(C=C(C=C4)Cl)Cl | |
| InChIKey | JCSGFHVFHSKIJH-UHFFFAOYSA-N |
Biological Activity
SB216763 is a potent, ATP-competitive inhibitor of glycogen synthase kinase-3 (GSK-3), inhibiting GSK-3α with an IC50 ofand possessing a Ki of 9 nM. By inhibiting GSK-3 activity, SB216763 blocks target phosphorylation, induces autophagy, and promotes glycogen synthesis in Chang liver cells with an EC50 of, thereby regulating downstream Wnt/β-catenin and metabolic signaling pathways. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
23 mg/mL
(61.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
23 mg/mL
(61.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 1 mg/mL |
| Water | Insoluble |
| DMSO |
23 mg/mL
(61.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
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Note: Technical data last updated: Sep 1, 2026.