CAS No. 112887-68-0

Batch: Purity:99.92
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Raltitrexed Chemical Structure
Chemical Structure
Check the Raltitrexed product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S)-2-[[5-[methyl-[(2-methyl-4-oxo-3H-quinazolin-6-yl)methyl]amino]thiophene-2-carbonyl]amino]pentanedioic acid
CAS Number 112887-68-0
Molecular Formula

C21H22N4O6S
Molecular Weight (MW) 458.49
SMILES CC1=NC2=C(C=C(C=C2)CN(C)C3=CC=C(S3)C(=O)NC(CCC(=O)O)C(=O)O)C(=O)N1
InChIKey IVTVGDXNLFLDRM-HNNXBMFYSA-N

Biological Activity

Raltitrexed is a folate antimetabolite and potent thymidylate synthase inhibitor that inhibits L1210 cell growth with an IC50 of 9 nM. Following cellular uptake, raltitrexed undergoes intracellular polyglutamation by folylpolyglutamate synthetase, markedly increasing its affinity for thymidylate synthase. Blockage of this pathway depletes thymidine triphosphate, disrupting DNA synthesis and inducing cell death.[1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 92 mg/mL (200.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Jackman AL, et al. Cancer Res. 1991;51(20):5579-5586.

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Note: Technical data last updated: Sep 1, 2026.