CAS No. 927880-90-8

Batch: Purity:99.98
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RAF265 (CHIR-265) Chemical Structure
Chemical Structure
Check the RAF265 (CHIR-265) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-methyl-5-[[2-[5-(trifluoromethyl)-1H-imidazol-2-yl]-4-pyridinyl]oxy]-N-[4-(trifluoromethyl)phenyl]benzimidazol-2-amine
CAS Number 927880-90-8
Molecular Formula

C24H16F6N6O
Molecular Weight (MW) 518.41
SMILES CN1C2=C(C=C(C=C2)OC3=CC(=NC=C3)C4=NC=C(N4)C(F)(F)F)N=C1NC5=CC=C(C=C5)C(F)(F)F
InChIKey YABJJWZLRMPFSI-UHFFFAOYSA-N

Ordering Information

Biological Activity

RAF265 (CHIR-265) is a potent, selective small-molecule inhibitor that targets C-Raf, B-Raf, B-Raf V600E, and VEGFR2. By blocking RAF kinase activity and VEGFR2 autophosphorylation, RAF265 suppresses downstream RAS/RAF/MEK/ERK signaling and angiogenic pathways. This targeted inhibition disrupts cellular signal transduction, ultimately resulting in cell cycle arrest, inhibition of cell proliferation, and the induction of apoptosis in dependent tumor cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (192.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 33 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.