CAS No. 114-80-7

Batch: Purity:99.69
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Neostigmine Bromide Chemical Structure
Chemical Structure
Check the Neostigmine Bromide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name [3-(dimethylcarbamoyloxy)phenyl]-trimethylazanium bromide
CAS Number 114-80-7
Molecular Formula

C12H19N2O2.Br
Molecular Weight (MW) 303.2
SMILES CN(C)C(=O)OC1=CC=CC(=C1)[N+](C)(C)C.[Br-]
InChIKey LULNWZDBKTWDGK-UHFFFAOYSA-M

Biological Activity

Neostigmine bromide is a reversible acetylcholinesterase inhibitor and parasympathomimetic agent. By blocking the active site and anionic site of acetylcholinesterase, it prevents the enzymatic degradation of endogenous acetylcholine in the synaptic cleft. This inhibition leads to increased accumulation of acetylcholine, indirectly activating both nicotinic and muscarinic acetylcholine receptors to restore cholinergic neurotransmission and facilitate neuromuscular signaling. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 60 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Sussman JL, et al. Science. 1991;253(5022):872-9.

[2] Soreq H, et al. Nat Rev Neurosci. 2001;2(4):294-302.

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Note: Technical data last updated: Sep 1, 2026.