CAS No. 13010-47-4

Batch: Purity:99.77
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Lomustine Chemical Structure
Chemical Structure
Check the Lomustine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea
CAS Number 13010-47-4
Molecular Formula

C9H16ClN3O2
Molecular Weight (MW) 233.7
SMILES C1CCC(CC1)NC(=O)N(CCCl)N=O
InChIKey GQYIWUVLTXOXAJ-UHFFFAOYSA-N

Biological Activity

Lomustine is a lipophilic nitrosourea alkylating agent that functions as a DNA-damaging compound. Upon undergoing spontaneous hydrolysis in physiological conditions, it generates reactive electrophilic intermediates that induce DNA alkylation and interstrand DNA cross-linking. These DNA modifications disrupt nucleic acid template function, resulting in the inhibition of DNA synthesis and cell division. Consequently, lomustine suppresses tumor cell proliferation across various cell-based models of hematologic malignancies and solid tumors. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 47 mg/mL (201.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Kaina B, et al. DNA Repair (Amst). 2007;6(8):1079-99.

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Note: Technical data last updated: Sep 1, 2026.