CAS No. 479-41-4

Batch: Purity:99.07
Check CoA
Indirubin (NSC 105327) Chemical Structure
Chemical Structure
Check the Indirubin (NSC 105327) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-(2-hydroxy-1H-indol-3-yl)indol-3-one
CAS Number 479-41-4
Molecular Formula

C16H10N2O2
Molecular Weight (MW) 262.26
SMILES C1=CC=C2C(=C1)C(=C(N2)O)C3=NC4=CC=CC=C4C3=O
InChIKey JNLNPCNGMHKCKO-UHFFFAOYSA-N

Ordering Information

Biological Activity

Indirubin is a potent inhibitor of cyclin-dependent kinases (CDKs) and glycogen synthase kinase-3β (GSK-3β). By blocking CDK- and GSK-3β-mediated tau phosphorylation, it disrupts aberrant hyperphosphorylation pathways involved in neurodegenerative disease. Additionally, indirubin suppresses tumor necrosis factor (TNF)-induced NF-κB activation, inhibiting IκB degradation and p65 nuclear translocation to impair cancer cell proliferation and promote apoptosis. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
1 mg 5 mg 10 mg
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 21.875 mg/mL (83.4 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.

Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.

Jump to

Note: Technical data last updated: Sep 1, 2026.