How to Prepare Stock Solution?
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Check the
Hydrocortisone (Cortisol)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
72 mg/mL
(198.64 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 15 mg/mL |
| Water | Insoluble |
| DMSO |
72 mg/mL
(198.64 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 12 mg/mL |
| Water | Insoluble |
| DMSO |
72 mg/mL
(198.64 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 9 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Storage
| Form | Storage Temp | Shelf Life |
|---|---|---|
| Powder | -20°C | 3 years |
| In Solvent | -80°C | 1 year |
| In Solvent | -20°C | 1 month |
Working Concentrations
Search directly for a cell line, or select a cell line to find matched working concentrations
Jump to
- Hydrocortisone (Cortisol) Mechanism of Action: [NF-κB] Inhibition in [Macrophages] Research
- Hydrocortisone (Cortisol) Clinical Trials
- Hydrocortisone (Cortisol) Working Concentration | Cell Culture | Treatment
- Hydrocortisone (Cortisol) Solubility in DMSO
- Hydrocortisone (Cortisol) Storage
- Hydrocortisone (Cortisol) Stability
- Hydrocortisone (Cortisol) Combination Database
- Hydrocortisone (Cortisol) Molecular Weight
- Hydrocortisone (Cortisol) SMILES
- Hydrocortisone (Cortisol) CAS Number
- Hydrocortisone (Cortisol) Chemical Structure (2D and 3D)
- Hydrocortisone (Cortisol) IC50 for Cell-based and Cell-free Assays
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- Hydrocortisone (Cortisol) SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.