CAS No. 864082-47-3

Batch: Purity:99.92
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GSK429286A Chemical Structure
Chemical Structure
Check the GSK429286A product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-(6-fluoro-1H-indazol-5-yl)-6-methyl-2-oxo-4-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1H-pyridine-5-carboxamide
CAS Number 864082-47-3
Molecular Formula

C21H16F4N4O2
Molecular Weight (MW) 432.37
SMILES CC1=C(C(CC(=O)N1)C2=CC=C(C=C2)C(F)(F)F)C(=O)NC3=C(C=C4C(=C3)C=NN4)F
InChIKey OLIIUAHHAZEXEX-UHFFFAOYSA-N

Biological Activity

GSK429286A is a cell-permeable, selective Rho-associated kinase (ROCK) inhibitor targeting ROCK1 and ROCK2. By inhibiting ROCK kinase activity, it modulates actin cytoskeleton organization and regulates cell adhesion, migration, and contraction. Furthermore, inhibition of the ROCK signaling pathway prevents dissociation-induced apoptosis and significantly improves the survival and cloning efficiency of human embryonic stem cells while preserving their pluripotency and self-renewal capabilities. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 86 mg/mL (198.9 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 5 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Watanabe K, et al. Nat Biotechnol. 2007;25(6):681-6.

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Note: Technical data last updated: Sep 1, 2026.