CAS No. 147-24-0

Batch: Purity:99.50
Check CoA
Diphenhydramine HCl Chemical Structure
Chemical Structure
Check the Diphenhydramine HCl product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-benzhydryloxy-N,N-dimethylethanamine;hydrochloride
CAS Number 147-24-0
Molecular Formula

C17H21NO.HCl
Molecular Weight (MW) 291.82
SMILES CN(C)CCOC(C1=CC=CC=C1)C2=CC=CC=C2.Cl
InChIKey PCHPORCSPXIHLZ-UHFFFAOYSA-N

Ordering Information

Biological Activity

Diphenhydramine HCl is a classic first-generation histamine H1 receptor antagonist that competitively blocks histamine binding to H1 receptors. By inhibiting H1 receptor-mediated signal transduction, it suppresses histamine-induced phosphoinositide hydrolysis, intracellular calcium mobilization, and downstream NF-kB pathway activation. Consequently, this competitive antagonism reduces histamine-driven cellular responses, vascular permeability, and inflammatory tissue phenotypes. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 58 mg/mL (198.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 58 mg/mL
Ethanol 58 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.

Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.

Jump to

Note: Technical data last updated: Sep 1, 2026.