CAS No. 56-75-7

Batch: Purity:99.97
Check CoA
Chloramphenicol Chemical Structure
Chemical Structure
Check the Chloramphenicol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide
CAS Number 56-75-7
Molecular Formula

C11H12Cl2N2O5
Molecular Weight (MW) 323.13
SMILES C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
InChIKey WIIZWVCIJKGZOK-RKDXNWHRSA-N

Biological Activity

Chloramphenicol is a broad-spectrum bacteriostatic antibiotic that targets the 50S ribosomal subunit. It selectively binds to the 23S rRNA within the peptidyl transferase center of bacterial ribosomes, effectively blocking peptidyl transferase activity. By preventing peptide bond formation during translation, chloramphenicol inhibits bacterial protein synthesis, leading to the arrest of bacterial cell growth across a wide range of Gram-positive, Gram-negative, and anaerobic microorganisms. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 65 mg/mL (201.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 65 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.

Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.

Reference

[1] Dunkle JA, et al. Proc Natl Acad Sci U S A. 2010;107(40):17152-7.

Jump to

Note: Technical data last updated: Sep 1, 2026.