CAS No. 88321-09-9

Batch: Purity:99.83
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Aloxistatin (E-64d) Chemical Structure
Chemical Structure
Check the Aloxistatin (E-64d) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name ethyl (2S,3S)-3-[[(2S)-4-methyl-1-(3-methylbutylamino)-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylate
CAS Number 88321-09-9
Molecular Formula

C17H30N2O5
Molecular Weight (MW) 342.43
SMILES CCOC(=O)C1C(O1)C(=O)NC(CC(C)C)C(=O)NCCC(C)C
InChIKey SRVFFFJZQVENJC-IHRRRGAJSA-N

Ordering Information

Biological Activity

Aloxistatin (E-64d) is a cell-permeable, irreversible inhibitor of cysteine proteases, including cathepsins B and L and calpains. By inhibiting intracellular cysteine proteases, aloxistatin blocks downstream proteolytic degradation pathways, impairs blood platelet aggregation, and suppresses viral processing. Consequently, aloxistatin markedly reduces cathepsin-dependent cellular entry of SARS-CoV-2 pseudovirions and restricts cathepsin-mediated protein cleavage in preclinical models. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 68 mg/mL (198.58 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 68 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.