CAS No. 41294-56-8

Batch: Purity:99.97
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Alfacalcidol Chemical Structure
Chemical Structure
Check the Alfacalcidol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name trans-(1R,3S,5Z)-5-[(2E)-2-[(1R,3aS,7aR)-7a-methyl-1-[(2R)-6-methylheptan-2-yl]-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol
CAS Number 41294-56-8
Molecular Formula

C27H44O2
Molecular Weight (MW) 400.64
SMILES CC(C)CCCC(C)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C
InChIKey OFHCOWSQAMBJIW-AVJTYSNKSA-N

Biological Activity

Alfacalcidol is a synthetic analogue of vitamin D that acts as a non-selective vitamin D receptor (VDR) activator. Upon metabolic conversion to calcitriol, it binds to VDR and regulates calcium and phosphate homeostasis, stimulating intestinal calcium absorption and promoting bone mineralization. In preclinical models, alfacalcidol treatment inhibits bone resorption, suppresses bone loss, and enhances bone mineral density, supporting skeletal structure and function. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (199.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 80 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Calcif Tissue Int. 2000;66(4):315-315.

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Note: Technical data last updated: Sep 1, 2026.