CAS No. 60-31-1

Batch: Purity:98.89
Check CoA
Acetylcholine Chloride Chemical Structure
Chemical Structure
Check the Acetylcholine Chloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-acetyloxyethyl(trimethyl)azanium chloride
CAS Number 60-31-1
Molecular Formula

C7H16NO2.Cl
Molecular Weight (MW) 181.66
SMILES CC(=O)OCC[N+](C)(C)C.[Cl-]
InChIKey JUGOREOARAHOCO-UHFFFAOYSA-M

Biological Activity

Acetylcholine chloride is an endogenous neurotransmitter that acts as a natural agonist for both nicotinic and muscarinic acetylcholine receptors. Activation of these cholinergic receptors regulates vital signal transduction pathways in the central and peripheral nervous systems, modulating cardiac rate, smooth muscle contraction, glandular secretion, and synaptic plasticity. [1][2]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 36 mg/mL (198.17 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 36 mg/mL
Ethanol 36 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Nat Rev Drug Discov. 2003;2(1):3-3.

[2] Dani JA, et al. Annu Rev Pharmacol Toxicol. 2007;47:699-729.

Jump to

Note: Technical data last updated: Sep 1, 2026.