Molecular Weight: 386.65 g/mol

5alpha-Cholestan-3-one Chemical Structure
Chemical Structure
Check the 5alpha-Cholestan-3-one product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Ethanol 60 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Chemical Information

CAS Number 566-88-1
Molecular Formula

C27H46O
Molecular Weight (MW) 386.65
SMILES CC(C)CCCC(C)C1CCC2C1(CCC3C2CCC4C3(CCC(=O)C4)C)C

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Note: Technical data last updated: Sep 1, 2026.