CAS No. 566-88-1

Batch: Purity:98
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5alpha-Cholestan-3-one Chemical Structure
Chemical Structure
Check the 5alpha-Cholestan-3-one product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 566-88-1
Molecular Formula

C27H46O
Molecular Weight (MW) 386.65
SMILES CC(C)CCCC(C)C1CCC2C1(CCC3C2CCC4C3(CCC(=O)C4)C)C

Ordering Information

Biological Activity

5alpha-Cholestan-3-one is an endogenous sterol metabolite and a substrate for cholestenone 5alpha-reductase involved in steroid biosynthesis and lipid metabolic pathways. As an intermediate in 3-oxosteroid conversion, it participates in enzymatic reduction processes that regulate cellular cholesterol utilization, bile acid biosynthesis, and steroid hormone turnover, thereby modulating lipid homeostasis and associated downstream signaling cascades in metabolic tissue systems.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Ethanol 60 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.