CAS No. 566-65-4

Batch: Purity:98.06
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5a-Pregnane-3,20-dione Chemical Structure
Chemical Structure
Check the 5a-Pregnane-3,20-dione product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (5S,8R,9S,10S,13S,14S,17S)-17-acetyl-10,13-dimethyl-1,2,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-3-one
CAS Number 566-65-4
Molecular Formula
C21H32O2
Molecular Weight (MW) 316.48
InChIKey XMRPGKVKISIQBV-BJMCWZGWSA-N

Ordering Information

Biological Activity

5a-Pregnane-3,20-dione (5α-dihydroprogesterone) is an endogenous progesterone metabolite involved in regulating cytoskeletal organization and cell adhesion pathways. The compound actively depolymerizes F-actin and downregulates the expression of key structural proteins, specifically actin and vinculin. Through these alterations in cytoskeletal integrity and adhesion molecules, 5a-Pregnane-3,20-dione promotes breast cell proliferation, migration, neoplasia, and invasive metastatic phenotypes.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Ethanol 3.5 mg/mL
DMSO 3 mg/mL (9.47 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.