Molecular Weight: 142.11 g/mol

5-Hydroxymethyluracil Chemical Structure
Chemical Structure
Check the 5-Hydroxymethyluracil product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

How to Prepare Stock Solution?

Concentration Volume Mass
1 mg 5 mg 10 mg
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 28 mg/mL (197.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Chemical Information

CAS Number 4433-40-3
Molecular Formula

C5H6N2O3
Molecular Weight (MW) 142.11
SMILES C1=C(C(=O)NC(=O)N1)CO

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Note: Technical data last updated: Sep 1, 2026.