CAS No. 4433-40-3
Batch:
Purity:99.77
Chemical Structure
Check the
5-Hydroxymethyluracil
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 4433-40-3 | |
|---|---|---|
| Molecular Formula |
C5H6N2O3 |
|
| Molecular Weight (MW) | 142.11 | |
| SMILES | C1=C(C(=O)NC(=O)N1)CO |
5-Hydroxymethyluracil is a modified pyrimidine base present in genomic DNA across diverse organisms, serving as a key derivative formed during pyrimidine oxidation. It acts as an endogenous DNA modification processed by the base excision repair pathway, primarily recognized and removed by the DNA glycosylase SMUG1 to maintain genomic stability. Accumulation of this modified base triggers repair responses and modulates epigenetic signaling dynamics in eukaryotic nucleic acids. [1]
|
Concentration
Volume
Mass
|
1 mg | 5 mg | 10 mg |
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
28 mg/mL
(197.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.