5-Fluorouracil (5-FU) is a non-selective thymidylate synthase (TS) and DHFR inhibitor. It affects PI3K/AKT/mTOR, p53, and NF-κB signaling pathways, by forming an inactive ternary complex with TS and incorporating into nucleic acids, inhibiting DNA synthesis and promoting apoptosis and cell cycle arrest.
5-Fluorouracil (5-FU) IC50 for Cell-based and Cell-free Assays
Comprehensive IC50 Values for 5-Fluorouracil (5-FU) Across Cell-free Enzyme Inhibitory Profiles and Cell-based Functional Assays.