CAS No. 53-85-0

Batch: Purity:99.73
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5,6-Dichlorobenzimidazole 1-beta-D-ribofuranoside Chemical Structure
Chemical Structure
Check the 5,6-Dichlorobenzimidazole 1-beta-D-ribofuranoside product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2R,3R,4S,5R)-2-(5,6-dichlorobenzimidazol-1-yl)-5-(hydroxymethyl)oxolane-3,4-diol
CAS Number 53-85-0
Molecular Formula

C12H12Cl2N2O4
Molecular Weight (MW) 319.14
InChIKey XHSQDZXAVJRBMX-DDHJBXDOSA-N

Ordering Information

Biological Activity

5,6-Dichlorobenzimidazole riboside (DRB) is a nucleoside analog that inhibits carboxyl-terminal domain (CTD) kinases, including casein kinase II and cyclin-dependent kinases (CDKs) such as CDK7 and CDK9. By blocking RNA polymerase II during transcription elongation, DRB inhibits RNA synthesis and triggers p53-dependent apoptosis in human colon adenocarcinoma cells without inducing genotoxic stress in healthy cells.[1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 64 mg/mL (200.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 5 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.